
A quick, but less specific measure of protein concentration is the absorbance of the sample at. Add ice-cold lysis buffer (1 mL per 100 mg or 100 µL of wet cell pellet). Western Blotting: Protein Quantification Absorbance at 280 nm. Pellet cells by centrifugation at 2,500 x g for 10 minutes. Wash the cells once by resuspending the cell pellet in ice-cold PBS.

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Wb quantification registration#
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